Publications

@article{Bonilla-Diaz2023-eh,

title = {Differentiated epithelial cells of the gut},

author = {Andrea Bonilla-D\'{i}az and Paloma Ord\'{o}\~{n}ez-Mor\'{a}n},

year  = {2023},

date = {2023-01-01},

journal = {Methods Mol. Biol.},

volume = {2650},

pages = {3\textendash16},

abstract = {The intestine is a prime example of self-renewal where stem cells 

 give rise to progenitor cells called transit-amplifying cells 

 which differentiate into more specialized cells. There are two 

 intestinal lineages: the absorptive (enterocytes and microfold 

 cells) and the secretory (Paneth cells, enteroendocrine, goblet 

 cells, and tuft cells). Each of these differentiated cell types 

 has a role in creating an ``ecosystem'' to maintain intestinal 

 homeostasis. Here, we summarize the main roles of each cell type.},

keywords = {Colon, Differentiation, Enterocytes, Enteroendocrine cells, Goblet cells, Intestine, Microfold cells, Paneth cells, Tuft   cells},

pubstate = {published},

tppubtype = {article}

}

@article{Gajera2023-kn,

title = {In vitro and in vivo assays for testing retinoids effect on 

 intestinal progenitors' lineage commitments},

author = {Krishna R Gajera and Kathryn L Fair and Gordon W Moran and Nicholas R F Hannan and Joerg Huelsken and Paloma Ord\'{o}\~{n}ez-Mor\'{a}n},

year  = {2023},

date = {2023-01-01},

journal = {Methods Mol. Biol.},

volume = {2650},

pages = {53\textendash61},

abstract = {The intestine consists of epithelial cells surrounded by a 

 complex environment as mesenchymal cells and the gut microbiota. 

 With its impressive stem cell regeneration capability, the 

 intestine is able to constantly replenish cells lost through 

 apoptosis or abrasion by food passing through. Over the past 

 decade, researchers have identified signaling pathways involved 

 in stem cell homeostasis such as retinoids pathway. Retinoids are 

 also involved in cell differentiation of healthy and cancer 

 cells. In this study, we describe several approaches in vitro and 

 in vivo to further investigate the effect of retinoids on stem 

 cells, progenitors, and differentiated intestinal cells.},

keywords = {Absorptive, Differentiation, Intestine, Organoids, Retinoids, Secretory, Stem cells},

pubstate = {published},

tppubtype = {article}

}

@article{Marva2023-zw,

title = {Novel approach to measure transepithelial electrical resistance 

 in intestinal cells},

author = {Gurveer Marva and Seyda \"{U}nsal and Andrew V Benest and David O Bates and Paloma Ord\'{o}\~{n}ez-Mor\'{a}n},

year  = {2023},

date = {2023-01-01},

journal = {Methods Mol. Biol.},

volume = {2650},

pages = {35\textendash42},

abstract = {The technique electric cell-substrate impedance sensing (ECIS) 

 can be used to detect and monitor the behavior of intestinal 

 cells. The methodology presented was designed to achieve results 

 within a short time frame, and it was tailored to use a colonic 

 cancer cell line. Differentiation of intestinal cancer cells has 

 previously been reported to be regulated by retinoic acid (RA). 

 Here, colonic cancer cells were cultured in the ECIS array before 

 being treated with RA, and any changes in response to RA were 

 monitored after treatment. The ECIS recorded changes in impedance 

 in response to the treatment and vehicle. This methodology poses 

 as a novel way to record the behavior of colonic cells and opens 

 new avenues for in vitro research.},

keywords = {Caco-2 cells, Colon, Differentiation, ECIS, Intestine, TEER},

pubstate = {published},

tppubtype = {article}

}