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Marva, Gurveer; Ünsal, Seyda; Benest, Andrew V; Bates, David O; Ordóñez-Morán, Paloma
Novel approach to measure transepithelial electrical resistance in intestinal cells Journal Article
In: Methods Mol. Biol., vol. 2650, pp. 35–42, 2023.
Abstract | Tags: Caco-2 cells, Colon, Differentiation, ECIS, Intestine, TEER
@article{Marva2023-zw,
title = {Novel approach to measure transepithelial electrical resistance
in intestinal cells},
author = {Gurveer Marva and Seyda \"{U}nsal and Andrew V Benest and David O Bates and Paloma Ord\'{o}\~{n}ez-Mor\'{a}n},
year = {2023},
date = {2023-01-01},
journal = {Methods Mol. Biol.},
volume = {2650},
pages = {35\textendash42},
abstract = {The technique electric cell-substrate impedance sensing (ECIS)
can be used to detect and monitor the behavior of intestinal
cells. The methodology presented was designed to achieve results
within a short time frame, and it was tailored to use a colonic
cancer cell line. Differentiation of intestinal cancer cells has
previously been reported to be regulated by retinoic acid (RA).
Here, colonic cancer cells were cultured in the ECIS array before
being treated with RA, and any changes in response to RA were
monitored after treatment. The ECIS recorded changes in impedance
in response to the treatment and vehicle. This methodology poses
as a novel way to record the behavior of colonic cells and opens
new avenues for in vitro research.},
keywords = {Caco-2 cells, Colon, Differentiation, ECIS, Intestine, TEER},
pubstate = {published},
tppubtype = {article}
}
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