Publications

@article{Martinez-Espuga2023-gt,

title = {Intestinal cell differentiation and phenotype in 2D and 3D 

 cell culture models},

author = {Magda Mart\'{i}nez-Espuga and Alvaro Mata and Paloma Ord\'{o}\~{n}ez-Mor\'{a}n},

year  = {2023},

date = {2023-01-01},

journal = {Methods Mol. Biol.},

volume = {2650},

pages = {235\textendash243},

abstract = {Three-dimensional (3D) culture models are more physiologically 

 relevant than two-dimensional (2D) cell culture models. 2D 

 approaches cannot reproduce the complexity of the tumor 

 microenvironment and are less able to translate biological 

 insights; and drug response studies have many limitations to be 

 extrapolated to the clinics. Here, we use the Caco-2 colon cancer 

 cell line, which is an immortalized human epithelial cell line 

 that under specific conditions can polarize and differentiate 

 into a villus-like phenotype. We describe cell differentiation 

 and cell growth in both 2D and 3D culture conditions, concluding 

 that cell morphology, polarity, proliferation and differentiation 

 are highly dependent on the type of cell culture system.},

keywords = {2D, 3D, Caco-2 cells, Differentiation, Gene expression, Markers},

pubstate = {published},

tppubtype = {article}

}

@article{Marva2023-zw,

title = {Novel approach to measure transepithelial electrical resistance 

 in intestinal cells},

author = {Gurveer Marva and Seyda \"{U}nsal and Andrew V Benest and David O Bates and Paloma Ord\'{o}\~{n}ez-Mor\'{a}n},

year  = {2023},

date = {2023-01-01},

journal = {Methods Mol. Biol.},

volume = {2650},

pages = {35\textendash42},

abstract = {The technique electric cell-substrate impedance sensing (ECIS) 

 can be used to detect and monitor the behavior of intestinal 

 cells. The methodology presented was designed to achieve results 

 within a short time frame, and it was tailored to use a colonic 

 cancer cell line. Differentiation of intestinal cancer cells has 

 previously been reported to be regulated by retinoic acid (RA). 

 Here, colonic cancer cells were cultured in the ECIS array before 

 being treated with RA, and any changes in response to RA were 

 monitored after treatment. The ECIS recorded changes in impedance 

 in response to the treatment and vehicle. This methodology poses 

 as a novel way to record the behavior of colonic cells and opens 

 new avenues for in vitro research.},

keywords = {Caco-2 cells, Colon, Differentiation, ECIS, Intestine, TEER},

pubstate = {published},

tppubtype = {article}

}