Publications

@article{Martinez-Espuga2023-gt,

title = {Intestinal cell differentiation and phenotype in 2D and 3D 

 cell culture models},

author = {Magda Mart\'{i}nez-Espuga and Alvaro Mata and Paloma Ord\'{o}\~{n}ez-Mor\'{a}n},

year  = {2023},

date = {2023-01-01},

journal = {Methods Mol. Biol.},

volume = {2650},

pages = {235\textendash243},

abstract = {Three-dimensional (3D) culture models are more physiologically 

 relevant than two-dimensional (2D) cell culture models. 2D 

 approaches cannot reproduce the complexity of the tumor 

 microenvironment and are less able to translate biological 

 insights; and drug response studies have many limitations to be 

 extrapolated to the clinics. Here, we use the Caco-2 colon cancer 

 cell line, which is an immortalized human epithelial cell line 

 that under specific conditions can polarize and differentiate 

 into a villus-like phenotype. We describe cell differentiation 

 and cell growth in both 2D and 3D culture conditions, concluding 

 that cell morphology, polarity, proliferation and differentiation 

 are highly dependent on the type of cell culture system.},

keywords = {2D, 3D, Caco-2 cells, Differentiation, Gene expression, Markers},

pubstate = {published},

tppubtype = {article}

}