Publications

@article{Qiu2023-np,

title = {Elucidating osseointegration in vivo in 3D printed scaffolds 

 eliciting different foreign body responses},

author = {Dewei Qiu and Chuanliang Cao and Aruna Prasopthum and Zhenchang Sun and Shan Zhang and Hanwen Yang and Zhiyong Xu and Jun Tao and Fanrong Ai and Jing Yang},

year  = {2023},

date = {2023-10-01},

journal = {Mater. Today Bio},

volume = {22},

number = {100771},

pages = {100771},

publisher = {Elsevier BV},

abstract = {Osseointegration between biomaterial and bone is critical for 

 the clinical success of many orthopaedic and dental implants. 

 However, the mechanisms of in vivo interfacial bonding formation 

 and the role of immune cells in this process remain unclear. In 

 this study, we investigated the bone-scaffold material 

 interfaces in two different 3D printed porous scaffolds 

 (polymer/hydroxyapatite and sintered hydroxyapatite) that 

 elicited different levels of foreign body response (FBR). The 

 polymer/hydroxyapatite composite scaffolds elicited more 

 intensive FBR, which was evidenced by more FBR components, such 

 as macrophages/foreign body giant cells and fibrous tissue, 

 surrounding the material surface. Sintered hydroxyapatite 

 scaffolds showed less intensive FBR compared to the composite 

 scaffolds. The interfacial bonding appeared to form via new bone 

 first forming within the pores of the scaffolds followed by 

 growing towards strut surfaces. In contrast, it was previously 

 thought that bone regeneration starts at biomaterial surfaces 

 via osteogenic stem/progenitor cells first attaching to them. 

 The material-bone interface of the less immunogenic 

 hydroxyapatite scaffolds was heterogenous across all samples, 

 evidenced by the coexistence of osseointegration and FBR 

 components. The presence of FBR components appeared to inhibit 

 osseointegration. Where FBR components were present there was no 

 osseointegration. Our results offer new insight on the in vivo 

 formation of bone-material interface, which highlights the 

 importance of minimizing FBR to facilitate osseointegration for 

 the development of better orthopaedic and dental biomaterials.},

keywords = {3D printing, biomaterials, bone, Foreign body response, Osseointegration, Tissue engineering},

pubstate = {published},

tppubtype = {article}

}

@article{Dooley2020-vf,

title = {Investigating the feasibility of spatially offset Raman 

 spectroscopy for in-vivo monitoring of bone healing in rat 

 calvarial defect models},

author = {Max Dooley and Jane McLaren and Felicity R A J Rose and Ioan Notingher},

year  = {2020},

date = {2020-10-01},

journal = {J. Biophotonics},

volume = {13},

number = {10},

pages = {e202000190},

publisher = {Wiley},

abstract = {A wide range of biomaterials and tissue-engineered scaffolds are 

 being investigated to support and stimulate bone healing in 

 animal models. Using phantoms and rat cadavers, we investigated 

 the feasibility of using spatially offset Raman spectroscopy 

 (SORS) to monitor changes in collagen concentration at levels 

 similar to those expected to occur in vivo during bone 

 regeneration (0-0.84 g/cm3 ). A partial least squares (PLS) 

 regression model was developed to quantify collagen 

 concentration in plugs consisting of mixtures or collagen and 

 hydroxyapatite (predictive power of $±$0.16 g/cm3 ). The PLS 

 model was then applied on SORS spectra acquired from rat 

 cadavers after implanting the collagen: hydroxyapatite plugs in 

 drilled skull defects. The PLS model successfully predicting the 

 profile of collagen concentration, but with an increased 

 predictive error of $±$0.30 g/cm3 . These results demonstrate 

 the potential of SORS to quantify collagen concentrations, in 

 the range relevant to those occurring during new bone formation.},

keywords = {bone, connective tissue, regenerative medicine, SORS},

pubstate = {published},

tppubtype = {article}

}