Publications

@article{Dooley2020-vf,

title = {Investigating the feasibility of spatially offset Raman 

 spectroscopy for in-vivo monitoring of bone healing in rat 

 calvarial defect models},

author = {Max Dooley and Jane McLaren and Felicity R A J Rose and Ioan Notingher},

year  = {2020},

date = {2020-10-01},

journal = {J. Biophotonics},

volume = {13},

number = {10},

pages = {e202000190},

publisher = {Wiley},

abstract = {A wide range of biomaterials and tissue-engineered scaffolds are 

 being investigated to support and stimulate bone healing in 

 animal models. Using phantoms and rat cadavers, we investigated 

 the feasibility of using spatially offset Raman spectroscopy 

 (SORS) to monitor changes in collagen concentration at levels 

 similar to those expected to occur in vivo during bone 

 regeneration (0-0.84 g/cm3 ). A partial least squares (PLS) 

 regression model was developed to quantify collagen 

 concentration in plugs consisting of mixtures or collagen and 

 hydroxyapatite (predictive power of $±$0.16 g/cm3 ). The PLS 

 model was then applied on SORS spectra acquired from rat 

 cadavers after implanting the collagen: hydroxyapatite plugs in 

 drilled skull defects. The PLS model successfully predicting the 

 profile of collagen concentration, but with an increased 

 predictive error of $±$0.30 g/cm3 . These results demonstrate 

 the potential of SORS to quantify collagen concentrations, in 

 the range relevant to those occurring during new bone formation.},

keywords = {bone, connective tissue, regenerative medicine, SORS},

pubstate = {published},

tppubtype = {article}

}