Publications

@article{Nelson-Dummett2025-ea,

title = {Inkjet printed 3D architectures: from silver micropillar  arrays and lattices to multimaterial metamaterials},

author = {Oliver Nelson-Dummett and Thomas Whittaker and William Whittow and Jacek Wojcik and Juan Francisco Reyes Luna and Caitlin McCall and Ahmet Koca and Christopher J Tuck and Richard J M Hague and Lyudmila Turyanska},

doi = {10.1016/j.mtadv.2025.100584},

year  = {2025},

date = {2025-06-01},

urldate = {2025-06-01},

journal = {Mater. Today Adv.},

volume = {26},

number = {100584},

pages = {100584},

publisher = {Elsevier BV},

keywords = {3D printing, Multi-material 3D printing},

pubstate = {published},

tppubtype = {article}

}

@article{Qiu2023-np,

title = {Elucidating osseointegration in vivo in 3D printed scaffolds 

 eliciting different foreign body responses},

author = {Dewei Qiu and Chuanliang Cao and Aruna Prasopthum and Zhenchang Sun and Shan Zhang and Hanwen Yang and Zhiyong Xu and Jun Tao and Fanrong Ai and Jing Yang},

year  = {2023},

date = {2023-10-01},

journal = {Mater. Today Bio},

volume = {22},

number = {100771},

pages = {100771},

publisher = {Elsevier BV},

abstract = {Osseointegration between biomaterial and bone is critical for 

 the clinical success of many orthopaedic and dental implants. 

 However, the mechanisms of in vivo interfacial bonding formation 

 and the role of immune cells in this process remain unclear. In 

 this study, we investigated the bone-scaffold material 

 interfaces in two different 3D printed porous scaffolds 

 (polymer/hydroxyapatite and sintered hydroxyapatite) that 

 elicited different levels of foreign body response (FBR). The 

 polymer/hydroxyapatite composite scaffolds elicited more 

 intensive FBR, which was evidenced by more FBR components, such 

 as macrophages/foreign body giant cells and fibrous tissue, 

 surrounding the material surface. Sintered hydroxyapatite 

 scaffolds showed less intensive FBR compared to the composite 

 scaffolds. The interfacial bonding appeared to form via new bone 

 first forming within the pores of the scaffolds followed by 

 growing towards strut surfaces. In contrast, it was previously 

 thought that bone regeneration starts at biomaterial surfaces 

 via osteogenic stem/progenitor cells first attaching to them. 

 The material-bone interface of the less immunogenic 

 hydroxyapatite scaffolds was heterogenous across all samples, 

 evidenced by the coexistence of osseointegration and FBR 

 components. The presence of FBR components appeared to inhibit 

 osseointegration. Where FBR components were present there was no 

 osseointegration. Our results offer new insight on the in vivo 

 formation of bone-material interface, which highlights the 

 importance of minimizing FBR to facilitate osseointegration for 

 the development of better orthopaedic and dental biomaterials.},

keywords = {3D printing, biomaterials, bone, Foreign body response, Osseointegration, Tissue engineering},

pubstate = {published},

tppubtype = {article}

}

@article{Hamid2021-mr,

title = {3D bioprinting of a stem cell-laden, multi-material tubular 

 composite: An approach for spinal cord repair},

author = {Omar A Hamid and Hoda M Eltaher and Virginie Sottile and Jing Yang},

year  = {2021},

date = {2021-01-01},

journal = {Mater. Sci. Eng. C Mater. Biol. Appl.},

volume = {120},

number = {111707},

pages = {111707},

publisher = {Elsevier BV},

abstract = {Development of a biomimetic tubular scaffold capable of 

 recreating developmental neurogenesis using pluripotent stem 

 cells offers a novel strategy for the repair of spinal cord 

 tissues. Recent advances in 3D printing technology have 

 facilitated biofabrication of complex biomimetic environments by 

 precisely controlling the 3D arrangement of various acellular 

 and cellular components (biomaterials, cells and growth 

 factors). Here, we present a 3D printing method to fabricate a 

 complex, patterned and embryoid body (EB)-laden tubular scaffold 

 composed of polycaprolactone (PCL) and hydrogel (alginate or 

 gelatine methacrylate (GelMA)). Our results revealed 3D printing 

 of a strong, macro-porous PCL/hydrogel tubular scaffold with a 

 high capacity to control the porosity of the PCL scaffold, 

 wherein the maximum porosity in the PCL wall was 15%. The 

 method was equally employed to create spatiotemporal protein 

 concentration within the scaffold, demonstrating its ability to 

 generate linear and opposite gradients of model molecules 

 (fluorescein isothiocyanate-conjugated bovine serum albumin 

 (FITC-BSA) and rhodamine). 3D bioprinting of EBs-laden GelMA was 

 introduced as a novel 3D printing strategy to incorporate EBs in 

 a hydrogel matrix. Cell viability and proliferation were 

 measured post-printing. Following the bioprinting of EBs-laden 

 5% GelMA hydrogel, neural differentiation of EBs was induced 

 using 1 μM retinoic acid (RA). The differentiated EBs 

 contained βIII-tubulin positive neurons displaying axonal 

 extensions and cells migration. Finally, 3D bioprinting of 

 EBs-laden PCL/GelMA tubular scaffold successfully supported EBs 

 neural differentiation and patterning in response to co-printing 

 with 1 μM RA. 3D printing of a complex heterogeneous tubular 

 scaffold that can encapsulate EBs, spatially controlled protein 

 concentration and promote neuronal patterning will help in 

 developing more biomimetic scaffolds capable of replicating the 

 neural patterning which occurs during neural tube development.},

keywords = {3D printing, Embryoid body (EB), Gradient, Hydrogels, Nerve   regeneration, Neural differentiation, Polycaprolactone},

pubstate = {published},

tppubtype = {article}

}

@article{Bagley2020-af,

title = {Low-cost automated vectors and modular environmental sensors for 

 plant phenotyping},

author = {Stuart A Bagley and Jonathan A Atkinson and Henry Hunt and Michael H Wilson and Tony P Pridmore and Darren M Wells},

year  = {2020},

date = {2020-06-01},

journal = {Sensors (Basel)},

volume = {20},

number = {11},

pages = {3319},

publisher = {MDPI AG},

abstract = {High-throughput plant phenotyping in controlled environments 

 (growth chambers and glasshouses) is often delivered via large, 

 expensive installations, leading to limited access and the 

 increased relevance of ``affordable phenotyping'' solutions. We 

 present two robot vectors for automated plant phenotyping under 

 controlled conditions. Using 3D-printed components and 

 readily-available hardware and electronic components, these 

 designs are inexpensive, flexible and easily modified to 

 multiple tasks. We present a design for a thermal imaging robot 

 for high-precision time-lapse imaging of canopies and a Plate 

 Imager for high-throughput phenotyping of roots and shoots of 

 plants grown on media plates. Phenotyping in controlled 

 conditions requires multi-position spatial and temporal 

 monitoring of environmental conditions. We also present a 

 low-cost sensor platform for environmental monitoring based on 

 inexpensive sensors, microcontrollers and internet-of-things 

 (IoT) protocols.},

keywords = {3D printing, IoT sensors, phenomics vectors, phenotyping robots},

pubstate = {published},

tppubtype = {article}

}

@article{Ruiz-Cantu2020-ep,

title = {Multi-material 3D bioprinting of porous constructs for 

 cartilage regeneration},

author = {Laura Ruiz-Cantu and Andrew Gleadall and Callum Faris and Joel Segal and Kevin Shakesheff and Jing Yang},

year  = {2020},

date = {2020-04-01},

journal = {Mater. Sci. Eng. C Mater. Biol. Appl.},

volume = {109},

number = {110578},

pages = {110578},

publisher = {Elsevier BV},

abstract = {The current gold standard for nasal reconstruction after 

 rhinectomy or severe trauma includes transposition of autologous 

 cartilage grafts in conjunction with coverage using an 

 autologous skin flap. Harvesting autologous cartilage requires a 

 major additional procedure that may create donor site morbidity. 

 Major nasal reconstruction also requires sculpting autologous 

 cartilages to form a cartilage framework, which is complex, 

 highly skill-demanding and very time consuming. These 

 limitations have prompted facial reconstructive surgeons to 

 explore different techniques such as tissue engineered 

 cartilage. This work explores the use of multi-material 3D 

 bioprinting with chondrocyte-laden gelatin methacrylate (GelMA) 

 and polycaprolactone (PCL) to fabricate constructs that can 

 potentially be used for nasal reconstruction. In this study, we 

 have investigated the effect of 3D manufacturing parameters 

 including temperature, needle gauge, UV exposure time, and cell 

 carrier formulation (GelMA) on the viability and functionality 

 of chondrocytes in bioprinted constructs. Furthermore, we 

 printed chondrocyte-laden GelMA and PCL into composite 

 constructs to combine biological and mechanical properties. It 

 was found that 20% w/v GelMA was the best concentration for the 

 3D bioprinting of the chondrocytes without comprising the 

 scaffold's porous structure and cell functionality. In addition, 

 the 3D bioprinted constructs showed neocartilage formation and 

 similar mechanical properties to nasal alar cartilage after a 

 50-day culture period. Neocartilage formation was also observed 

 in the composite constructs evidenced by the presence of 

 glycosaminoglycans and collagen type II. This study shows the 

 feasibility of manufacturing neocartilage using 

 chondrocytes/GelMA/PCL 3D bioprinted porous constructs which 

 could be applied as a method for fabricating implants for nose 

 reconstruction.},

keywords = {3D printing, Bioprinting, Cartilage, Chondrocytes, GelMA, Multi-material 3D printing, Polycaprolactone, Surface porosity, Tissue engineering},

pubstate = {published},

tppubtype = {article}

}