Publications

@article{Tarantini2022-zb,

title = {Direct RT-qPCR assay for the detection of SARS-CoV-2 in 

 saliva samples},

author = {Francesco Saverio Tarantini and Siyu Wu and Harry Jenkins and Ana Tellechea Lopez and Hannah Tomlin and Ralph Hyde and Katarzyna Lis-Slimak and Jamie Louise Thompson and Sara Pijuan-Galit\'{o} and Danielle Scales and Kazuyo Kaneko and Jayasree Dey and Emily Park and Jack Hill and I-Ning Lee and Lara Doolan and Asta Arendt-Tranholm and Chris Denning and Claire Seedhouse and Andrew V Benest},

year  = {2022},

date = {2022-03-01},

journal = {Methods Protoc.},

volume = {5},

number = {2},

pages = {25},

publisher = {MDPI AG},

abstract = {Since mid-2020 there have been complexities and difficulties in 

 the standardisation and administration of nasopharyngeal swabs. 

 Coupled with the variable and/or poor accuracy of lateral flow 

 devices, this has led to increased societal 'testing fatigue' 

 and reduced confidence in test results. Consequently, 

 asymptomatic individuals have developed reluctance towards 

 repeat testing, which remains the best way to monitor COVID-19 

 cases in the wider population. On the other hand, saliva-based 

 PCR, a non-invasive, highly sensitive, and accurate test 

 suitable for everyone, is gaining momentum as a straightforward 

 and reliable means of detecting SARS-CoV-2 in symptomatic and 

 asymptomatic individuals. Here, we provide an itemised list of 

 the equipment and reagents involved in the process of sample 

 submission, inactivation and analysis, as well as a detailed 

 description of how each of these steps is performed.},

keywords = {COVID-19, qPCR, saliva},

pubstate = {published},

tppubtype = {article}

}