Publications

@article{Owen2024-ih,

title = {β-glycerophosphate, not low magnitude fluid shear stress,  increases osteocytogenesis in the osteoblast-to-osteocyte cell  line IDG-SW3},

author = {Robert Owen and Claudia Wittkowske and Damien Lacroix and Cecile M Perrault and Gwendolen C Reilly},

doi = {10.1080/03008207.2024.2375065},

year  = {2024},

date = {2024-07-01},

urldate = {2024-07-01},

journal = {Connect. Tissue Res.},

volume = {65},

number = {4},

pages = {313\textendash329},

publisher = {Informa UK Limited},

abstract = {AIM: As osteoblasts deposit a mineralized collagen network, a 

 subpopulation of these cells differentiates into osteocytes. 

 Biochemical and mechanical stimuli, particularly fluid shear 

 stress (FSS), are thought to regulate this, but their relative 

 influence remains unclear. Here, we assess both biochemical and 

 mechanical stimuli on long-term bone formation and 

 osteocytogenesis using the osteoblast-osteocyte cell line 

 IDG-SW3. METHODS: Due to the relative novelty and uncommon 

 culture conditions of IDG-SW3 versus other osteoblast-lineage 

 cell lines, effects of temperature and media formulation on 

 matrix deposition and osteocytogenesis were initially 

 characterized. Subsequently, the relative influence of 

 biochemical (β-glycerophosphate (βGP) and ascorbic 

 acid 2-phosphate (AA2P)) and mechanical stimulation on 

 osteocytogenesis was compared, with intermittent application of 

 low magnitude FSS generated by see-saw rocker. RESULTS: 

 βGP and AA2P supplementation were required for 

 mineralization and osteocytogenesis, with 33°C cultures 

 retaining a more osteoblastic phenotype and 37°C cultures 

 undergoing significantly higher osteocytogenesis. βGP 

 concentration positively correlated with calcium deposition, 

 whilst AA2P stimulated alkaline phosphatase (ALP) activity and 

 collagen deposition. We demonstrate that increasing βGP 

 concentration also significantly enhances osteocytogenesis as 

 quantified by the expression of green fluorescent protein linked 

 to Dmp1. Intermittent FSS (~0.06 Pa) rocker had no effect on 

 osteocytogenesis and matrix deposition. CONCLUSIONS: This work 

 demonstrates the suitability and ease with which IDG-SW3 can be 

 utilized in osteocytogenesis studies. IDG-SW3 mineralization was 

 only mediated through biochemical stimuli with no detectable 

 effect of low magnitude FSS. Osteocytogenesis of IDG-SW3 

 primarily occurred in mineralized areas, further demonstrating 

 the role mineralization of the bone extracellular matrix has in 

 osteocyte differentiation.},

keywords = {biomechanics, bone tissue engineering, extracellular matrix, Matrix mineralization, mechanobiology},

pubstate = {published},

tppubtype = {article}

}