Publications

@article{Woodhouse2023-wp,

title = {Quantification of drugs in brain and liver mimetic tissue models 

 using Raman spectroscopy},

author = {Nathan Woodhouse and Jan Majer and Peter Marshall and Steve Hood and Ioan Notingher},

year  = {2023},

date = {2023-03-01},

journal = {Appl. Spectrosc.},

volume = {77},

number = {3},

pages = {246\textendash260},

publisher = {SAGE Publications},

abstract = {Quantitative analysis of drug delivery with in biological 

 systems is an integral challenge in drug development. Analytical 

 techniques are important for assessing both drug target 

 delivery, target action, and drug toxicology. Using mimetic 

 tissue models, we have investigated the efficacy of Raman 

 spectroscopy in quantitative detection of alkyne group and 

 deuterated drugs in rat brain and rat liver tissue models. 

 Lasers with 671 nm and 785 nm wavelengths were assessed for 

 their feasibility in this application due to opposing relative 

 benefits and disadvantages. Thin tissue sections have been 

 tested as a practical means of reducing autofluorescent 

 background by minimizing out-of-focus tissue and therefore 

 maximizing photobleaching rates. Alkyne-tagged drugs were 

 quantitatively measured at 18 $±$ 5 μg/g drug/tissue mass 

 ratio in rat brain and at 34 $±$ 6 μg/g in rat liver. 

 Quantification calibration curves were generated for a range of 

 concentrations from 0-500 μg/g. These results show the 

 potential of Raman spectroscopy as a diffraction-limited 

 spatially resolved imaging technique for assessing drug delivery 

 in tissue applications.},

keywords = {Drug delivery, drug quantification, microscopy, Raman spectroscopy},

pubstate = {published},

tppubtype = {article}

}