Publications

@article{Kocon2025-ay,

title = {Regional profiling reveals a distinct glioblastoma infiltrative  margin proteome},

author = {Artur Kocon and Stuart J Smith and Benito Morentin and Luis F Callado and Wayne Carter and Ruman Rahman},

doi = {10.1038/s41598-025-09228-z},

year  = {2025},

date = {2025-07-01},

urldate = {2025-07-01},

journal = {Sci. Rep.},

volume = {15},

number = {1},

pages = {24021},

publisher = {Springer Science and Business Media LLC},

abstract = {Isocitrate dehydrogenase wild-type glioblastoma, a malignant 

 brain tumour of glial origin, confers a poor prognosis with a 

 median survival of 12 to 16 months from diagnosis. Glioblastomas 

 are aggressive tumours that rapidly proliferate and diffusely 

 infiltrate surrounding brain tissue. Current multimodal standard 

 treatment is typically ineffective and despite gross total 

 surgical resection, tumours recur with more aggressive 

 sub-clonal populations of malignant cells. A defining 

 characteristic of glioblastoma is its highly heterogeneous 

 nature and acquirement of somatic mutations advantageous to 

 tumour growth and suppression of apoptotic pathways. 

 Pathogenesis of malignant brain tumours as well as its mode of 

 transformation to a more aggressive subtype is still largely 

 unknown. Although genomic studies have elucidated a plethora of 

 genetic markers associated with glioblastoma subtypes, only a 

 few have been utilised in a clinical setting. One of the 

 emerging approaches to studying glioblastomas is by 

 investigating how an active proteome contributes to its 

 aggressive nature. Furthermore, through activation of specific 

 pathways via post-translational modifications of proteins such 

 as phosphorylation, glioblastomas create an intricate network of 

 signalling pathways which favour tumour growth and 

 proliferation. Here, we investigated the feasibility of diverse 

 methodological approaches to describe abnormal protein 

 signalling across distinct intra-tumour regions of primary 

 glioblastoma tissue, including proliferative core, peripheral 

 rim, and invasive margin. Whilst we observe a broadly comparable 

 proteome relative to the human non-diseased brain, we identify 

 cytoplasmic proteins α-trypsin, actin, apolipoprotein A1 

 and transthyretin which may putatively be associated with the 

 GBM infiltrative tumour margin.},

keywords = {2D-PAGE, Glioblastoma, Proteomics, Tandem mass spectrometry},

pubstate = {published},

tppubtype = {article}

}