Publications

@article{Burgess2020-ss,

title = {Covalent attachment of active enzymes to upconversion phosphors 

 allows ratiometric detection of substrates},

author = {Letitia Burgess and Hannah Wilson and Alex R Jones and Peter Harvey and Louise S Natrajan and Sam Hay},

year  = {2020},

date = {2020-11-01},

journal = {Chemistry},

volume = {26},

number = {65},

pages = {14817\textendash14822},

publisher = {Wiley},

abstract = {Upconverting phosphors (UCPs) convert multiple low energy 

 photons into higher energy emission via the process of photon 

 upconversion and offer an attractive alternative to organic 

 fluorophores for use as luminescent probes. Here, UCPs were 

 capped with functionalized silica in order to provide a surface 

 to covalently conjugate proteins with surface-accessible 

 cysteines. Variants of green fluorescent protein (GFP) and the 

 flavoenzyme pentaerythritol tetranitrate reductase (PETNR) were 

 then attached via maleimide-thiol coupling in order to allow 

 energy transfer from the UCP to the GFP or flavin cofactor of 

 PETNR, respectively. PETNR retains its activity when coupled to 

 the UCPs, which allows reversible detection of enzyme substrates 

 via ratiometric sensing of the enzyme redox state.},

keywords = {biosensors, energy transfer, enzymes, lanthanides, upconversion},

pubstate = {published},

tppubtype = {article}

}