Publications

@article{Islam2022-yy,

title = {Evolution of silicate bioglass particles as porous microspheres 

 with a view towards orthobiologics},

author = {Md Towhidul Islam and Nur Aisyah Nuzulia and Laura Macri-Pellizzeri and Farah Nigar and Yessie W Sari and Ifty Ahmed},

year  = {2022},

date = {2022-03-01},

journal = {J. Biomater. Appl.},

volume = {36},

number = {8},

pages = {1427\textendash1443},

publisher = {SAGE Publications},

abstract = {Although FDA approved and clinically utilised, research on 45S5 

 Bioglass® and S53P4 including other bioactive 

 glasses continues in order to advance their applicability for a 

 range of alternate applications. For example, rendering these 

 particles porous would enable incorporation of varying 

 biological payloads (i.e. cells, drugs and growth factors) and 

 making them spherical would enhance their flow properties 

 enabling delivery to target sites via minimally invasive 

 injection procedures. This paper reports on the manufacture of 

 solid (non-porous; SGMS) and highly porous microspheres (PGMS) 

 with large external pores and fully interconnected porosity from 

 bioactive silicate glass formulations (45S5 and S53P4) via a 

 single stage flame spheroidisation process and their 

 physicochemical properties including in vitro biological 

 response. Morphological and physical characterisation of the 

 SGMS and PGMS revealed interconnected porosity up to 65 $±$ 

 5%. Mass loss studies comparing between SGMS and PGMS revealed 

 1.5 times higher mass loss for the PGMS over 28 days. Also, in 

 vitro bioactivity studies using simulated body fluid (SBF) 

 revealed hydroxyapatite (HA) formation at earlier time point for 

 PGMS compared to their SGMS counterparts (i.e day 1 for PGMS and 

 day 3 for SGMS of 45S5). In addition, HA layers were also formed 

 in cell culture media, with the exception of SGMS of 45S5, which 

 revealed CaP formation with a ratio of 1.52-1.78. Direct cell 

 seeding and indirect cell culture studies (via incubation with 

 microsphere degradation products) revealed mouse 3T3 cells were 

 able to grow and undergo osteogenic differentiation in vitro, 

 confirming cytocompatibility of both 45S5 and S53P4 SGMS and 

 PGMS. More importantly and especially for orthobiologic 

 applications, cells were observed to have migrated within the 

 pores of the PGMS. As such, the PGMS developed from these 

 bioactive silicate glasses are highly promising candidate 

 materials for orthobiologics and alternate applications 

 requiring delivery of biologic payloads.},

keywords = {bioactive glasses, bioactivity, cytocompatibility, mass loss, orthobiologics, osteogenic differentiation, porous microspheres, thermal properties},

pubstate = {published},

tppubtype = {article}

}