Publications

@article{Figueiredo2020-bz,

title = {Quantifying oxygen levels in 3D bioprinted cell-laden thick 

 constructs with perfusable microchannel networks},

author = {Lara Figueiredo and Catherine Le Visage and Pierre Weiss and Jing Yang},

year  = {2020},

date = {2020-05-01},

journal = {Polymers (Basel)},

volume = {12},

number = {6},

pages = {1260},

publisher = {MDPI AG},

abstract = {The survival and function of thick tissue engineered implanted 

 constructs depends on pre-existing, embedded, functional, 

 vascular-like structures that are able to integrate with the 

 host vasculature. Bioprinting was employed to build perfusable 

 vascular-like networks within thick constructs. However, the 

 improvement of oxygen transportation facilitated by these 

 vascular-like networks was directly quantified. Using an optical 

 fiber oxygen sensor, we measured the oxygen content at different 

 positions within 3D bioprinted constructs with and without 

 perfusable microchannel networks. Perfusion was found to play an 

 essential role in maintaining relatively high oxygen content in 

 cell-laden constructs and, consequently, high cell viability. 

 The concentration of oxygen changes following switching on and 

 off the perfusion. Oxygen concentration depletes quickly after 

 pausing perfusion but recovers rapidly after resuming the 

 perfusion. The quantification of oxygen levels within cell-laden 

 hydrogel constructs could provide insight into channel network 

 design and cellular responses.},

keywords = {3D bioprinting, hydrogel, microchannels, microfluidics, oxygen, silated-HPMC},

pubstate = {published},

tppubtype = {article}

}