Publications

@article{Owen2020-gt,

title = {Comparison of the anabolic effects of reported osteogenic 

 compounds on human mesenchymal progenitor-derived osteoblasts},

author = {Robert Owen and Hossein Bahmaee and Frederik Claeyssens and Gwendolen C Reilly},

year  = {2020},

date = {2020-01-01},

journal = {Bioengineering (Basel)},

volume = {7},

number = {1},

pages = {12},

publisher = {MDPI AG},

abstract = {There is variability in the reported effects of compounds on 

 osteoblasts arising from differences in experimental design and 

 choice of cell type/origin. This makes it difficult to discern a 

 compound's action outside its original study and compare 

 efficacy between compounds. Here, we investigated five compounds 

 frequently reported as anabolic for osteoblasts 

 (17β-estradiol (oestrogen), icariin, lactoferrin, lithium 

 chloride, and menaquinone-4 (MK-4)) on human mesenchymal 

 progenitors to assess their potential for bone tissue 

 engineering with the aim of identifying a potential alternative 

 to expensive recombinant growth factors such as bone 

 morphogenetic protein 2 (BMP-2). Experiments were performed 

 using the same culture conditions to allow direct comparison. 

 The concentrations of compounds spanned two orders of magnitude 

 to encompass the reported efficacious range and were applied 

 continuously for 22 days. The effects on the proliferation 

 (resazurin reduction and DNA quantification), osteogenic 

 differentiation (alkaline phosphatase (ALP) activity), and 

 mineralised matrix deposition (calcium and collagen 

 quantification) were assessed. Of these compounds, only 10 µM 

 MK-4 stimulated a significant anabolic response with 50% 

 greater calcium deposition. Oestrogen and icariin had no 

 significant effects, with the exception of 1 µM icariin, which 

 increased the metabolic activity on days 8 and 22. 1000 µg/mL of 

 lactoferrin and 10 mM lithium chloride both significantly 

 reduced the mineralised matrix deposition in comparison to the 

 vehicle control, despite the ALP activity being higher in 

 lithium chloride-treated cells at day 15. This demonstrates that 

 MK-4 is the most powerful stimulant of bone formation in hES-MPs 

 of the compounds investigated, highlighting its potential in 

 bone tissue engineering as a method of promoting bone formation, 

 as well as its prospective use as an osteoporosis treatment.},

keywords = {bone formation, bone tissue engineering, matrix mineralisation, menaquinone-4, mesenchymal stem cells, osteoblasts, osteoporosis, vitamin K},

pubstate = {published},

tppubtype = {article}

}