Publications

@article{Sinjab2020-az,

title = {Induction and measurement of the early stage of a host-parasite 

 interaction using a combined optical trapping and Raman 

 microspectroscopy system},

author = {Faris Sinjab and Hany M Elsheikha and Max Dooley and Ioan Notingher},

year  = {2020},

date = {2020-02-01},

journal = {J. Biophotonics},

volume = {13},

number = {2},

pages = {e201960065},

publisher = {Wiley},

abstract = {Understanding and quantifying the temporal acquisition of host 

 cell molecules by intracellular pathogens is fundamentally 

 important in biology. In this study, a recently developed 

 holographic optical trapping (HOT)-based Raman microspectroscopy 

 (RMS) instrument is applied to detect, characterize and monitor 

 in real time the molecular trafficking of a specific molecular 

 species (isotope-labeled phenylalanine (L-Phe(D8)) at the single 

 cell level. This approach enables simultaneous measurement of 

 the chemical composition of human cerebrovascular endothelial 

 cells and the protozoan parasite Toxoplasma gondii in isolation 

 at the very start of the infection process. Using a model to 

 decouple measurement contributions from host and pathogen 

 sampling in the excitation volume, the data indicate that 

 manipulating parasites with HOT coupled with RMS chemical 

 readout was an effective method for measurement of L-Phe(D8) 

 transfer from host cells to parasites in real-time, from the 

 moment the parasite enters the host cell.},

keywords = {blood-brain-barrier, host-parasite interaction, optical trapping, Raman spectroscopy, Toxoplasma gondii},

pubstate = {published},

tppubtype = {article}

}