Publications

@article{Almozyan2021-jt,

title = {FLYWCH1, a multi-functional zinc finger protein contributes to 

 the DNA repair pathway},

author = {Sheema Almozyan and James Coulton and Roya Babaei-Jadidi and Abdolrahman S Nateri},

year  = {2021},

date = {2021-04-01},

journal = {Cells},

volume = {10},

number = {4},

pages = {889},

publisher = {MDPI AG},

abstract = {Over recent years, several Cys2-His2 (C2H2) domain-containing 

 proteins have emerged as critical players in repairing 

 DNA-double strand breaks. Human FLYWCH1 is a newly characterised 

 nuclear transcription factor with (C2H2)-type zinc-finger 

 DNA-binding domains. Yet, our knowledge about FLYWCH1 is still 

 in its infancy. This study explores the expression, role and 

 regulation of FLYWCH1 in the context of DNA damage and repair. 

 We provide evidence suggesting a potential contribution of 

 FLYWCH1 in facilitating the recruitment of DNA-damage response 

 proteins (DDRPs). We found that FLYWCH1 colocalises with 

 γH2AX in normal fibroblasts and colorectal cancer (CRC) 

 cell lines. Importantly, our results showed that enforced 

 expression of FLYWCH1 induces the expression of γH2AX, 

 ATM and P53 proteins. Using an ATM-knockout (ATMKO) model, we 

 indicated that FLYWCH1 mediates the phosphorylation of H2AX 

 (Ser139) independently to ATM expression. On the other hand, the 

 induction of DNA damage using UV-light induces the endogenous 

 expression of FLYWCH1. Conversely, cisplatin treatment reduces 

 the endogenous level of FLYWCH1 in CRC cell lines. Together, our 

 findings uncover a novel FLYWCH1/H2AX phosphorylation axis in 

 steady-state conditions and during the induction of the 

 DNA-damage response (DDR). Although the role of FLYWCH1 within 

 the DDR machinery remains largely uncharacterised and poorly 

 understood, we here report for the first-time findings that 

 implicate FLYWCH1 as a potential participant in the DNA damage 

 response signaling pathways.},

keywords = {ATM, CRC, Cys2-His2 (C2H2)-type zinc-finger, DNA-damage   response, FLYWCH1, p53, γH2AX},

pubstate = {published},

tppubtype = {article}

}