Publications

@article{Smith2021-jd,

title = {3D phonon microscopy with sub-micron axial-resolution},

author = {Richard J Smith and Fernando P\'{e}rez-Cota and Leonel Marques and Matt Clark},

year  = {2021},

date = {2021-02-01},

urldate = {2021-02-01},

journal = {Sci. Rep.},

volume = {11},

number = {1},

pages = {3301},

publisher = {Springer Science and Business Media LLC},

abstract = {Brillouin light scattering (BLS) is an emerging method for cell 

 imaging and characterisation. It allows elasticity-related 

 contrast, optical resolution and label-free operation. Phonon 

 microscopy detects BLS from laser generated coherent phonon 

 fields to offer an attractive route for imaging since, at GHz 

 frequencies, the phonon wavelength is sub-optical. Using phonon 

 fields to image single cells is challenging as the signal to 

 noise ratio and acquisition time are often poor. However, recent 

 advances in the instrumentation have enabled imaging of fixed 

 and living cells. This work presents the first experimental 

 characterisation of phonon-based axial resolution provided by 

 the response to a sharp edge. The obtained axial resolution is 

 up to 10 times higher than that of the optical system used to 

 take the measurements. Validation of the results are obtained 

 with various polymer objects, which are in good agreement with 

 those obtained using atomic force microscopy. Edge localisation, 

 and hence profilometry, of a phantom boundary is measured with 

 accuracy and precision of approximately 60 nm and 100 nm 

 respectively. Finally, 3D imaging of fixed cells in culture 

 medium is demonstrated.},

keywords = {3D imaging, microscopy, Phonon microscopy},

pubstate = {published},

tppubtype = {article}

}