Publications

@article{La_Cavera2024-oi,

title = {Label-free Brillouin endo-microscopy for the quantitative 3D  imaging of sub-micrometre biology},

author = {Salvatore La Cavera and Veeren M Chauhan and William Hardiman and Mengting Yao and Rafael Fuentes-Dom\'{i}nguez and Kerry Setchfield and Sidahmed A Abayzeed and Fernando P\'{e}rez-Cota and Richard J Smith and Matt Clark},

doi = {10.1038/s42003-024-06126-4},

year  = {2024},

date = {2024-04-01},

urldate = {2024-04-01},

journal = {Commun. Biol.},

volume = {7},

number = {1},

pages = {451},

abstract = {This report presents an optical fibre-based endo-microscopic 

 imaging tool that simultaneously measures the topographic profile 

 and 3D viscoelastic properties of biological specimens through 

 the phenomenon of time-resolved Brillouin scattering. This uses 

 the intrinsic viscoelasticity of the specimen as a contrast 

 mechanism without fluorescent tags or photoacoustic contrast 

 mechanisms. We demonstrate 2 μm lateral resolution and 320 nm 

 axial resolution for the 3D imaging of biological cells and 

 Caenorhabditis elegans larvae. This has enabled the first ever 3D 

 stiffness imaging and characterisation of the C. elegans larva 

 cuticle in-situ. A label-free, subcellular resolution, and 

 endoscopic compatible technique that reveals structural 

 biologically-relevant material properties of tissue could pave 

 the way toward in-vivo elasticity-based diagnostics down to the 

 single cell level.},

keywords = {Brillouin scattering, label free, microscopy},

pubstate = {published},

tppubtype = {article}

}